This week we had 4 inx pairs give with pr. 1 not making it through d1.

We observed a phenotype in all 3 pairs on d4 and 5.  However we did not see the same blood flow phenotype from a month earlier.  All three prs. exibited a small eye on d4 and d5.  On d7 these same pairs looked like they were dying and had more pigment then the non phenotype fish.

Again these fish were 100% red and were not sortable.

pr. 2.  9/30 observed phenotype.

pr. 3.  6/30 observed phenotype.

pr. 4.  8/30 observed phenotype.

This line needs to be tested and confirmed

This week we screened 4 inx pairs of gbt0433.  A phenotype was observed in all four pairs.  RFP was not sorted because inx pairs were 100% red (possibly maternal).  The phenotype was slow blood flow.  This phenotype was questionable as some did not agree or see the phenotype.

Pair. 1  7/30 contained phenotype

Pair. 2  1/31 contained phenotype

Pair. 3 8/30 contained phenotype

Pair. 4 16/27 contained phenotype

This phenotype needs to be reexamined with new inx. pairs and with other tools to measure blood flow.

26/35 RFP+ and no observed phenotype.