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Annotated Zebrafish Anatomical Ontology (ZFA) Expression Tags:
cardiovascular system, coelom, heart, trunk
Maternal mRFP: Y
Known Phenotypes: shorter body length at 8-10 dpf, reduced heart function, mitochondrial abnormal
Viability: Larval lethal at 12 dpf
Line Designation: GBT0425
Lab: Xu lab - Mayo Clinic
GBT Plasmid:
RP2.1
Tagged Gene: mrps18b; mitochondrial ribosomal protein S18B
(from GenBank)
Tagged Gene Status: Confirmed
Alternative Gene Names: wu:fj45h02, zgc:112517
(from GenBank)
Alternative Names: RP2_#385, ZIC10
Allele: xu0425GT
Insertion Location:
5' Genome:
GBT0425_5.txt 3' Genome:
GBT0425_3.txtRACE Tags: 5' RACE:
GBT0425_5R.txt 3' RACE:
Links: ZFIN Ensembl Entrez Gene
Additional Molecular Data
RFP Linkage Analysis:
Gene Primer: GAATAACGTCCATTGTCGCTCCGGTG
Trap Primer: CGCCCTTGGTCACCTTCAGCTT
Genotyping Primers:
Gene-P1: ATCTTCATCTGCTGATAACACC
Gene-P2: TTACATATCCCTGCACAACTTG
Trap-P3: GTACAGTAATCAAGTAAAATTACTCA
Genome Location:
Quantitative PCR Measurements: Knockdown Figure not provided
F primer: GAATAACGTCCATTGTCGCTCCGGTG
R primer: TGTCCACACAGGCTTTGTTCCATAGC
Trap primer:
Reference Gene:
MO Information
MO:
MO suggested dose:
MO phenotype(s):
No assay history for Henley lab
No assay history for Hyperosmotic Stress
No assay history for Morphological Phenotype
No assay history for Nicotine Biology
No assay history for tcap induction assay
Digital Notebook Entries:
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