Confirmation of genotyping primers
Attached is a gel picture of the confirmatory PCRs (Accidentally, the directionality of these primers at first so the F1 in the picture is actually the reverse primer and the R1 is the fwd primer.)
However, the primer sequences listed are correct. Additionally, the primers didn't work well later. The reverse primer is good (bright bands in picture) but the fwd had faint bands so possibly the PCR needs to be optimized better or fwd needs to be redesigned.